| 研究課題 |
①腫瘍ならびに正常細胞の放射線感受性関連遺伝子の同定とその機能解析ならびに細胞の放射線感受性の迅速な解析システムの開発
②低酸素腫瘍細胞での遺伝子発現変化についての解析
③腫瘍の温熱療法の基礎的検討
④DNA損傷の修復経路やDNA損傷と関連する細胞内シグナル伝達経路ならびにそれらと発癌との関連性の解析
⑤ヒトウイルソン病モデルラットを用いた活性酸素の産生と遺伝子損傷ならびに発癌との関連性と銅キレート剤の効果の検討
⑥老化促進マウスなどをもちいた脳における遺伝子損傷や老化における活性酸素の関与
⑦長波長紫外線など環境紫外線の生物作用
⑧血管新生抑制による腫瘍治療の基礎的研究 |
| 著書・論文など |
1. 林 正信 (2008) 腫瘍治療における血管新生と酸素効果のパラドックス、放射線生物研究、43(3)236-247
2. Hayashi, M., Nishiya, H., Chiba, T., Endoh, D., Kon, Y., and Okui, T. (2007) Trientine, A Copper-Chelating Agent, Induced Apoptosis in Murine Fibrosarcoma Cells in vivo and in vitro. J. Vet. Med. Sci. 69 (2) 137-142.
3. Hayashi, M., Hirai, R., Ishihara, Y., Horiguchi, N., Endoh, D. and Okui, T. (2007) Combined effects of treatment with trientine, a copper-chelating agent, and X-irradiaion on tumor growth in transplantation model of a murine fibrosarcoma. J. Vet. Med. Sci. 69 (10) 1039-1045.
4. 林 正信 (2006) 放射線誘発DNA2本鎖切断の修復における末端結合経路はいくつある？、Radioisotopes、55: 439-442.
5. 林 正信 (2006) Wilson病モデル動物（LECラット）を用いた病態解明と新しい治療戦略、医学のあゆみ、216(11): 832-836
6. Hayashi, M., Fuse, S., Endoh, D., Horiguchi, N., Nakayama, K., Kon, Y., Okui, T. 2006. Accumulation of copper induces DNA strand breaks in Brain cells of Long-Evans Cinnamon (LEC) rats, an animal model for human Wilson disease. Expt. Animals 55 (5):419-426.
7. Y. Kanae, Y., Endoh, D., Yamato, O., Hayashi, D,, Matsunaga, S., Ogawa, H., Maede, Y. and Hayashi, M. (2006) Nonsense mutation of feline β?hexosaminidase β?subunit (HEXB) gene causing Sandhoff disease in a pedigree of Japanese domestic cat. Res. Vet. Sci. 82 (1): 54-60.
8. Kanae, Y., Endoh, D., Yokota, H., Taniyama, H. Hayashi, M. (2006) Expression of the PTEN tumor suppressor gene in malignant mammary gland tumors of dogs. Am. J. Vet. Res. 67(1): 127-133.
9. Hayashi, M., Miyane, Y., Seno, M., Endoh, D., Higuchi, H., Nagahata, H., Kon, Y. and Okui, T. (2005) Inhibitory effects of trientine, a copper-chelating agent, on induction of DNA strand breaks in renal cells of Long-Evans Cinnamon (LEC) rats. Expt. Animals 54 (5):403-412.
10. Endoh, D., Mizutani, T., Kirisawa, R., Maki, Y., Hidetoshi Saito, Yasuhiro Kon, Shigeru Morikawa, Masanobu Hayashi (2005) Species-independent detection of RNA virus by representational difference analysis using non-ribosomal hexanucleotides for reverse transcription. Nucleic Acid Res. E65.
11. Kanae, Y., Endoh, D., Nagahata, H. and Hayashi, M. A method for detecting complex vertebral malformation in Holstein calves using polymerase chain reaction-primer introduced restrictrion analysis. J. Vet. Diagnostic Invest. 17: 258-262 (2005).
12. Hayashi, M., Miyane, T. Hirooka, D. Endoh, H. Higuchi, H. Nagahata, K. Nakayama, Y. Kon, and T. Okui, (2004) Inhibitory effects of trientine, a copper-chelating agent, on induction of DNA strand breaks in hepatic cells of Long-Evans Cinnamon (LEC) rats. Biochim. Biophys. Acta 1674(3): 312-318
13. Hayashi, M., Hamasu, T., Endoh, D., Kawana, I., Matsumoto, M. and Okui, T. (2004) High sensitivity of fibroblast cell lines derived from LEC rats to heat treatment. J. Vet. Med. Sci. 66(10) 1177-1182. |
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